NMR (Nuclear Magnetic Resonance) Spectroscopy
NMR (Nuclear Magnetic Resonance) Spectroscopy provides physical, chemical, electronic and structural information from organic compounds in liquid or solid form.
Samples are typically dissolved in a deuterium-labelled solvent to form, a clear solution before being transferred to a thin, transparent glass NMR tube. The sample is then placed into a very strong magnetic field whereby the nuclei of the atoms absorb and then re-emit electromagnetic radiation at a particular resonance frequency. This information provides structural and electronic information which translates into an extremely powerful analytical technique.
Nuclear Magnetic Resonance Spectroscopy offers:
- Identification of molecular chemical environments and structures
- Physical and chemical properties at the atomic and molecular level
- Well resolved, highly sensitive, analytically tractable and highly predictable analysis.
- Structural determination of molecules including biomacromolecules
- Determination of molecular motion and interaction profiles
- The study of proteins including enzyme active sites
- Compound identification
- Purity analysis
- Stability studies.
Typical Industries using NMR Spectroscopy:
- Pharmaceutical industry
- Agrochemical industry
- Chemical industry
- Polymer industry
- Medical device industry
- Nuclear industry
- Consumer health
- Packaging manufacturers
- Petrochemical and oil.
NMR Spectroscopy - At a Glance
- Information: Molecular information of simple and complex structures, normally relates to a single element
- Sample Prerequisites: Understanding the material so that appropriate solvent can be used as required
- Typical samples: Organic, inorganic or biochemical molecules
- Output: Separation by functional groups and differing neighbouring groups, plus relative quantities of elements present
- Sample Size: Sample sizes of less than 1g are required
- Detection Limits: NMR has limited sensitivity to minor components
- Data Output: Spectrum of signal intensity against chemical shift
- Elements Characterized: Analysis is normally limited to a single element, typically hydrogen
- Samples: Liquids, dissolved solids and solids may be analyzed using the appropriate instrumentation